The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for APPL1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = APPL1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of APPL1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:57,078,355–57,079,870 | 148.5 kb | Distal (>10kb) Multiome | 674 | |
| chr3:57,130,457–57,131,979 | 96.9 kb | Distal (>10kb) Multiome | 353 | |
| chr3:57,158,954–57,159,929 | 68.4 kb | Distal (>10kb) Multiome | 259 | |
| chr3:57,160,383–57,161,092 | 67.0 kb | Distal (>10kb) Multiome HiCAR | 147 | |
| chr3:57,164,404–57,166,039 | 62.4 kb | Distal (>10kb) Multiome HiCAR | 455 | |
| chr3:57,169,750–57,170,529 | 57.5 kb | Distal (>10kb) Multiome HiCAR | 491 | |
| chr3:57,194,399–57,195,064 | 33.0 kb | Distal (>10kb) Multiome | 31 | |
| chr3:57,199,761–57,200,263 | 27.7 kb | Distal (>10kb) Multiome | 256 | |
| chr3:57,227,073–57,228,173 | 83 bp | At TSS Multiome | 859 |
Genomic view of the APPL1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.