This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for APEH. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = APEH upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of APEH, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:49,411,409–49,412,844 | 262.2 kb | Distal (>10kb) Multiome | 899 | |
| chr3:49,422,179–49,422,905 | 251.8 kb | Distal (>10kb) Multiome | 453 | |
| chr3:49,428,857–49,429,869 | 244.9 kb | Distal (>10kb) Multiome | 712 | |
| chr3:49,461,484–49,462,134 | 212.6 kb | Distal (>10kb) Multiome | 207 | |
| chr3:49,468,926–49,470,930 | 204.2 kb | Distal (>10kb) Multiome HiCAR | 919 | |
| chr3:49,539,369–49,540,584 | 134.4 kb | Distal (>10kb) Multiome HiCAR | 628 | |
| chr3:49,553,618–49,555,244 | 119.9 kb | Distal (>10kb) Multiome HiCAR | 710 | |
| chr3:49,718,116–49,720,106 | 45.3 kb | Distal (>10kb) Multiome | 450 | |
| chr3:49,723,361–49,724,517 | 49.7 kb | Distal (>10kb) Multiome | 769 | |
| chr3:49,785,831–49,787,202 | 112.2 kb | Distal (>10kb) Multiome | 854 | |
| chr3:49,802,203–49,804,006 | 128.5 kb | Distal (>10kb) Multiome | 733 | |
| chr3:49,856,099–49,857,163 | 182.3 kb | Distal (>10kb) Multiome | 664 | |
| chr3:49,869,590–49,870,249 | 195.5 kb | Distal (>10kb) Multiome | 251 | |
| chr3:49,903,411–49,904,277 | 229.6 kb | Distal (>10kb) Multiome | 415 | |
| chr3:49,929,290–49,930,212 | 255.4 kb | Distal (>10kb) Multiome | 799 | |
| chr3:49,939,635–49,940,747 | 265.7 kb | Distal (>10kb) Multiome | 949 |
Genomic view of the APEH locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.