Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ANXA7. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ANXA7 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ANXA7, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:73,167,766–73,168,681 | 245.9 kb | Distal (>10kb) Multiome | 879 | |
| chr10:73,246,675–73,247,516 | 166.9 kb | Distal (>10kb) Multiome | 1023 | |
| chr10:73,252,167–73,253,067 | 161.4 kb | Distal (>10kb) Multiome | 929 | |
| chr10:73,358,383–73,358,977 | 55.3 kb | Distal (>10kb) Multiome | 468 | |
| chr10:73,413,302–73,414,453 | 18 bp | At TSS Multiome | 938 | |
| chr10:73,495,235–73,496,398 | 81.9 kb | Distal (>10kb) Multiome | 908 | |
| chr10:73,591,236–73,591,781 | 177.4 kb | Distal (>10kb) Multiome | 359 | |
| chr10:73,591,961–73,592,227 | 178.0 kb | Distal (>10kb) Multiome | 458 | |
| chr10:73,625,111–73,626,369 | 211.9 kb | Distal (>10kb) Multiome | 810 | |
| chr10:73,647,157–73,648,042 | 233.5 kb | Distal (>10kb) Multiome | 238 |
Genomic view of the ANXA7 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.