The Annexin 5 gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. Polymorphisms in this gene have been implicated in various obstetric complications. [provided by RefSeq, Dec 2019]
Transcription factors with Perturb-seq knockdown data for ANXA5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ANXA5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ANXA5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:121,514,259–121,514,846 | 182.3 kb | Distal (>10kb) Multiome | 104 | |
| chr4:121,688,123–121,688,889 | 8.1 kb | Proximal (<10kb) | 46 | |
| chr4:121,695,651–121,695,894 | 1.1 kb | Proximal (<10kb) | 46 | |
| chr4:121,696,158–121,697,590 | 24 bp | At TSS Multiome | 831 | |
| chr4:121,697,697–121,698,095 | 702 bp | At TSS | 45 | |
| chr4:121,711,374–121,712,252 | 14.7 kb | Distal (>10kb) Multiome | 592 | |
| chr4:121,749,014–121,749,684 | 52.4 kb | Distal (>10kb) Multiome | 138 | |
| chr4:121,764,589–121,765,761 | 68.1 kb | Distal (>10kb) Multiome | 279 | |
| chr4:121,800,778–121,801,755 | 104.3 kb | Distal (>10kb) Multiome | 866 | |
| chr4:121,822,774–121,824,390 | 127.0 kb | Distal (>10kb) Multiome | 880 | |
| chr4:121,869,547–121,871,130 | 173.5 kb | Distal (>10kb) Multiome | 687 | |
| chr4:121,900,129–121,900,683 | 203.5 kb | Distal (>10kb) Multiome | 142 | |
| chr4:121,950,003–121,952,427 | 253.4 kb | Distal (>10kb) Multiome | 598 |
Genomic view of the ANXA5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.