Transcription factors with Perturb-seq knockdown data for ANKRD40. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ANKRD40 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ANKRD40, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:50,425,595–50,426,626 | 281.4 kb | Distal (>10kb) Multiome | 619 | |
| chr17:50,478,411–50,479,262 | 228.8 kb | Distal (>10kb) Multiome | 956 | |
| chr17:50,508,047–50,508,856 | 199.2 kb | Distal (>10kb) Multiome | 332 | |
| chr17:50,541,672–50,542,697 | 165.5 kb | Distal (>10kb) Multiome HiCAR | 772 | |
| chr17:50,546,778–50,547,615 | 160.5 kb | Distal (>10kb) Multiome HiCAR | 786 | |
| chr17:50,558,590–50,561,781 | 147.1 kb | Distal (>10kb) Multiome | 587 | |
| chr17:50,586,525–50,587,142 | 120.9 kb | Distal (>10kb) Multiome | 43 | |
| chr17:50,634,652–50,635,325 | 72.6 kb | Distal (>10kb) Multiome | 471 | |
| chr17:50,707,179–50,708,006 | 11 bp | At TSS Multiome | 1055 | |
| chr17:50,719,167–50,720,018 | 11.9 kb | Distal (>10kb) Multiome | 1050 | |
| chr17:50,865,239–50,868,097 | 158.8 kb | Distal (>10kb) Multiome | 1256 | |
| chr17:50,905,047–50,905,768 | 197.9 kb | Distal (>10kb) Multiome | 556 | |
| chr17:50,931,004–50,931,866 | 223.6 kb | Distal (>10kb) Multiome | 780 | |
| chr17:50,944,102–50,945,190 | 237.0 kb | Distal (>10kb) Multiome | 985 | |
| chr17:50,950,043–50,950,638 | 242.9 kb | Distal (>10kb) Multiome | 713 |
Genomic view of the ANKRD40 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.