Transcription factors with Perturb-seq knockdown data for ANK2-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ANK2-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ANK2-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:112,706,097–112,706,856 | 274.0 kb | Distal (>10kb) Multiome | 313 | |
| chr4:112,746,884–112,747,846 | 233.3 kb | Distal (>10kb) Multiome | 275 | |
| chr4:112,751,041–112,752,361 | 228.8 kb | Distal (>10kb) Multiome | 200 | |
| chr4:112,759,125–112,759,682 | 221.0 kb | Distal (>10kb) Multiome | 195 | |
| chr4:112,817,726–112,818,715 | 162.3 kb | Distal (>10kb) Multiome | 463 | |
| chr4:112,854,045–112,854,900 | 126.0 kb | Distal (>10kb) Multiome | 101 | |
| chr4:112,860,814–112,861,553 | 119.4 kb | Distal (>10kb) Multiome | 167 | |
| chr4:112,979,338–112,980,523 | 535 bp | At TSS Multiome | 216 | |
| chr4:113,060,795–113,061,325 | 80.5 kb | Distal (>10kb) Multiome | 153 | |
| chr4:113,064,101–113,064,667 | 84.0 kb | Distal (>10kb) Multiome | 209 |
Genomic view of the ANK2-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.