AKAP8

A-kinase anchoring protein 8 | AKAP95, DKFZp586B1222

This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]

Member of: DE-5

Biological processes 31 terms

DNA binding (GO:0003677)NF-kappaB binding (GO:0051059)RNA binding (GO:0003723)cell cycle G2/M phase transition (GO:0044839)cellular response to lipopolysaccharide (GO:0071222)cellular response to prostaglandin E stimulus (GO:0071380)condensed chromosome (GO:0000793)cytoplasm (GO:0005737)double-stranded DNA binding (GO:0003690)female pronucleus (GO:0001939)histone deacetylase binding (GO:0042826)membrane (GO:0016020)mitotic cell cycle (GO:0000278)mitotic chromosome condensation (GO:0007076)negative regulation of tumor necrosis factor production (GO:0032720)nuclear lumen (GO:0031981)nuclear matrix (GO:0016363)nuclear matrix (GO:0016363)nuclear matrix (GO:0016363)nucleolus (GO:0005730)nucleolus (GO:0005730)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)protein binding (GO:0005515)protein kinase A regulatory subunit binding (GO:0034237)protein kinase A regulatory subunit binding (GO:0034237)protein kinase A regulatory subunit binding (GO:0034237)signal transduction (GO:0007165)zinc ion binding (GO:0008270)

Expression (TPM)

TFs regulating AKAP8 0 TFs

Transcription factors with Perturb-seq knockdown data for AKAP8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AKAP8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to AKAP8

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AKAP8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr19:15,106,942–15,107,576	272.5 kb	Distal (>10kb) Multiome	339
chr19:15,113,323–15,113,828	266.2 kb	Distal (>10kb) Multiome	116
chr19:15,124,546–15,126,107	254.1 kb	Distal (>10kb) Multiome	949
chr19:15,177,520–15,178,026	202.1 kb	Distal (>10kb) Multiome	499
chr19:15,180,886–15,181,623	198.6 kb	Distal (>10kb) Multiome	355
chr19:15,195,187–15,196,375	183.7 kb	Distal (>10kb) Multiome	408
chr19:15,199,629–15,201,437	179.7 kb	Distal (>10kb) Multiome	398
chr19:15,222,727–15,224,168	156.3 kb	Distal (>10kb) Multiome	521
chr19:15,231,632–15,233,858	146.6 kb	Distal (>10kb) Multiome	414
chr19:15,324,741–15,325,392	54.9 kb	Distal (>10kb) Multiome	183
chr19:15,331,471–15,332,992	47.3 kb	Distal (>10kb) Multiome	641
chr19:15,378,833–15,380,802	68 bp	At TSS Multiome	913
chr19:15,418,493–15,419,441	39.3 kb	Distal (>10kb) Multiome	892
chr19:15,432,448–15,433,787	53.1 kb	Distal (>10kb) Multiome	766
chr19:15,449,146–15,450,281	70.0 kb	Distal (>10kb) Multiome	446
chr19:15,451,962–15,453,427	72.7 kb	Distal (>10kb) Multiome	428
chr19:15,456,680–15,457,763	77.3 kb	Distal (>10kb) Multiome	240
chr19:15,555,803–15,556,689	176.2 kb	Distal (>10kb) Multiome	669
chr19:16,184,971–16,186,029	805.5 kb	Distal (>10kb) Multiome HiCAR	658

Genome Browser

Genomic view of the AKAP8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.