Transcription factors with Perturb-seq knockdown data for AGK-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AGK-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AGK-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:141,071,165–141,071,881 | 479.8 kb | Distal (>10kb) Multiome HiCAR | 334 | |
| chr7:141,072,522–141,074,991 | 476.8 kb | Distal (>10kb) Multiome HiCAR | 574 | |
| chr7:141,430,495–141,431,173 | 120.3 kb | Distal (>10kb) Multiome | 137 | |
| chr7:141,511,342–141,512,397 | 39.2 kb | Distal (>10kb) Multiome | 217 | |
| chr7:141,550,725–141,551,853 | 112 bp | At TSS Multiome | 899 | |
| chr7:141,584,907–141,585,342 | 33.8 kb | Distal (>10kb) Multiome | 149 | |
| chr7:141,701,501–141,702,649 | 150.7 kb | Distal (>10kb) Multiome | 455 | |
| chr7:141,737,828–141,738,838 | 187.0 kb | Distal (>10kb) Multiome | 1117 |
Genomic view of the AGK-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.