This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq, Oct 2008]
Transcription factors with Perturb-seq knockdown data for ADH5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ADH5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ADH5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:98,260,090–98,262,366 | 827.5 kb | Distal (>10kb) Multiome HiCAR | 990 | |
| chr4:98,800,757–98,802,020 | 287.3 kb | Distal (>10kb) Multiome | 287 | |
| chr4:98,927,921–98,930,006 | 159.5 kb | Distal (>10kb) Multiome | 1003 | |
| chr4:98,994,750–98,996,989 | 93.2 kb | Distal (>10kb) Multiome | 958 | |
| chr4:99,084,444–99,085,306 | 3.4 kb | Proximal (<10kb) | 89 | |
| chr4:99,085,426–99,085,751 | 3.0 kb | Proximal (<10kb) | 16 | |
| chr4:99,088,011–99,089,765 | 55 bp | At TSS Multiome | 875 | |
| chr4:99,091,353–99,091,515 | 2.6 kb | Proximal (<10kb) | 50 | |
| chr4:99,333,536–99,334,278 | 245.3 kb | Distal (>10kb) Multiome | 156 |
Genomic view of the ADH5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.