Angiogenesis is controlled by a local balance between stimulators and inhibitors of new vessel growth and is suppressed under normal physiologic conditions. Angiogenesis has been shown to be essential for growth and metastasis of solid tumors. In order to obtain blood supply for their growth, tumor cells are potently angiogenic and attract new vessels as results of increased secretion of inducers and decreased production of endogenous negative regulators. BAI1 contains at least one 'functional' p53-binding site within an intron, and its expression has been shown to be induced by wildtype p53. There are two other brain-specific angiogenesis inhibitor genes, designated BAI2 and BAI3 which along with BAI1 have similar tissue specificities and structures, however only BAI1 is transcriptionally regulated by p53. BAI1 is postulated to be a member of the secretin receptor family, an inhibitor of angiogenesis and a growth suppressor of glioblastomas [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ADGRB1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ADGRB1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ADGRB1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:142,402,231–142,404,149 | 140.3 kb | Distal (>10kb) Multiome | 776 | |
| chr8:142,447,487–142,447,963 | 1.7 kb | Proximal (<10kb) | 325 | |
| chr8:142,449,469–142,449,877 | at TSS | At TSS | 71 | |
| chr8:142,450,884–142,451,570 | 1.2 kb | Proximal (<10kb) | 159 | |
| chr8:142,452,045–142,453,565 | 90.6 kb | Distal (>10kb) Multiome | 400 | |
| chr8:142,471,794–142,472,417 | 71.3 kb | Distal (>10kb) Multiome | 323 | |
| chr8:142,474,787–142,476,474 | 67.8 kb | Distal (>10kb) Multiome | 367 | |
| chr8:142,613,900–142,615,241 | 71.0 kb | Distal (>10kb) Multiome | 485 |
Genomic view of the ADGRB1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.