Transcription factors with Perturb-seq knockdown data for ADD3-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ADD3-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ADD3-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:109,883,984–109,884,981 | 121.6 kb | Distal (>10kb) Multiome HiCAR | 460 | |
| chr10:109,922,853–109,924,531 | 82.5 kb | Distal (>10kb) Multiome | 670 | |
| chr10:109,934,169–109,935,521 | 70.9 kb | Distal (>10kb) Multiome | 274 | |
| chr10:110,000,884–110,001,244 | 7.1 kb | Proximal (<10kb) | 22 | |
| chr10:110,005,714–110,006,121 | 2.2 kb | Proximal (<10kb) | 182 | |
| chr10:110,006,797–110,009,028 | 1.9 kb | Proximal (<10kb) Multiome | 867 | |
| chr10:110,010,680–110,011,262 | 2.3 kb | Proximal (<10kb) | 86 | |
| chr10:110,016,047–110,016,356 | 7.7 kb | Proximal (<10kb) | 99 | |
| chr10:110,168,513–110,169,827 | 163.1 kb | Distal (>10kb) Multiome | 325 | |
| chr10:110,205,984–110,208,438 | 201.6 kb | Distal (>10kb) Multiome | 727 | |
| chr10:110,209,115–110,211,789 | 205.2 kb | Distal (>10kb) Multiome | 928 | |
| chr10:110,225,399–110,227,748 | 220.0 kb | Distal (>10kb) Multiome | 766 | |
| chr10:110,304,286–110,305,616 | 299.0 kb | Distal (>10kb) Multiome | 717 |
Genomic view of the ADD3-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.