This gene encodes a member of the acyl-CoA synthetase family of enzymes that activate fatty acids by catalyzing the formation of a thioester linkage between fatty acids and coenzyme A. The encoded protein is localized to mitochondria, has high specificity for malonate and methylmalonate and possesses malonyl-CoA synthetase activity. Mutations in this gene are a cause of combined malonic and methylmalonic aciduria. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Sep 2013]
Transcription factors with Perturb-seq knockdown data for ACSF3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ACSF3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ACSF3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:88,802,766–88,805,068 | 290.2 kb | Distal (>10kb) Multiome | 881 | |
| chr16:88,810,968–88,812,892 | 281.8 kb | Distal (>10kb) Multiome | 1059 | |
| chr16:88,856,080–88,858,003 | 236.9 kb | Distal (>10kb) Multiome | 885 | |
| chr16:88,940,964–88,942,110 | 152.0 kb | Distal (>10kb) Multiome | 323 | |
| chr16:89,093,591–89,094,129 | 52 bp | At TSS Multiome | 694 | |
| chr16:89,098,308–89,098,713 | 4.4 kb | Proximal (<10kb) | 340 | |
| chr16:89,167,237–89,167,783 | 73.7 kb | Distal (>10kb) Multiome | 740 | |
| chr16:89,170,902–89,172,416 | 77.7 kb | Distal (>10kb) Multiome | 464 | |
| chr16:89,200,493–89,202,260 | 107.9 kb | Distal (>10kb) Multiome | 598 | |
| chr16:89,217,337–89,217,960 | 123.8 kb | Distal (>10kb) Multiome | 857 | |
| chr16:89,240,678–89,241,104 | 147.1 kb | Distal (>10kb) Multiome | 864 | |
| chr16:89,246,875–89,247,970 | 153.7 kb | Distal (>10kb) Multiome | 740 | |
| chr16:89,290,889–89,291,526 | 197.2 kb | Distal (>10kb) Multiome | 171 | |
| chr16:89,360,791–89,361,256 | 267.1 kb | Distal (>10kb) Multiome | 194 |
Genomic view of the ACSF3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.