ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]
Transcription factors with Perturb-seq knockdown data for ACLY. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ACLY upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ACLY, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:41,647,765–41,648,495 | 270.9 kb | Distal (>10kb) Multiome | 519 | |
| chr17:41,662,549–41,663,015 | 256.1 kb | Distal (>10kb) Multiome | 643 | |
| chr17:41,665,564–41,667,291 | 251.8 kb | Distal (>10kb) Multiome | 883 | |
| chr17:41,688,103–41,690,064 | 230.1 kb | Distal (>10kb) Multiome | 1236 | |
| chr17:41,733,761–41,735,187 | 184.2 kb | Distal (>10kb) Multiome | 577 | |
| chr17:41,738,054–41,738,690 | 180.8 kb | Distal (>10kb) Multiome | 596 | |
| chr17:41,784,980–41,787,229 | 132.2 kb | Distal (>10kb) Multiome | 958 | |
| chr17:41,793,527–41,794,326 | 125.1 kb | Distal (>10kb) Multiome | 597 | |
| chr17:41,800,647–41,802,040 | 117.1 kb | Distal (>10kb) Multiome | 766 | |
| chr17:41,811,252–41,813,275 | 106.0 kb | Distal (>10kb) Multiome | 997 | |
| chr17:41,835,756–41,836,400 | 82.8 kb | Distal (>10kb) Multiome | 568 | |
| chr17:41,864,608–41,865,695 | 53.5 kb | Distal (>10kb) Multiome | 913 | |
| chr17:41,913,670–41,914,569 | 4.9 kb | Proximal (<10kb) Multiome | 190 | |
| chr17:41,918,026–41,919,794 | 213 bp | At TSS Multiome | 956 | |
| chr17:41,930,220–41,930,908 | 11.5 kb | Distal (>10kb) Multiome | 915 | |
| chr17:41,965,771–41,967,327 | 47.8 kb | Distal (>10kb) Multiome | 920 | |
| chr17:42,016,953–42,018,102 | 98.5 kb | Distal (>10kb) Multiome | 991 | |
| chr17:42,019,726–42,020,334 | 101.1 kb | Distal (>10kb) Multiome | 791 | |
| chr17:42,050,326–42,051,046 | 131.6 kb | Distal (>10kb) Multiome | 514 | |
| chr17:42,107,497–42,108,335 | 188.9 kb | Distal (>10kb) Multiome | 357 | |
| chr17:42,120,858–42,121,715 | 202.3 kb | Distal (>10kb) Multiome | 559 | |
| chr17:42,122,176–42,123,003 | 203.7 kb | Distal (>10kb) Multiome | 169 | |
| chr17:42,154,717–42,156,309 | 236.1 kb | Distal (>10kb) Multiome | 770 | |
| chr17:42,180,242–42,180,762 | 261.4 kb | Distal (>10kb) Multiome | 576 | |
| chr17:42,183,755–42,184,761 | 265.2 kb | Distal (>10kb) Multiome | 810 |
Genomic view of the ACLY locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.