The sphingolipid metabolite sphingosine-1-phosphate promotes cell proliferation and survival, whereas its precursor, sphingosine, has the opposite effect. The ceramidase ACER2 hydrolyzes very long chain ceramides to generate sphingosine (Xu et al., 2006 [PubMed 16940153]).[supplied by OMIM, Jul 2010]
Transcription factors with Perturb-seq knockdown data for ACER2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ACER2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ACER2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:19,126,915–19,128,564 | 281.5 kb | Distal (>10kb) Multiome | 808 | |
| chr9:19,229,553–19,231,458 | 178.5 kb | Distal (>10kb) Multiome | 973 | |
| chr9:19,379,591–19,380,878 | 28.7 kb | Distal (>10kb) Multiome | 1052 | |
| chr9:19,408,145–19,409,950 | 110 bp | At TSS Multiome | 773 | |
| chr9:19,446,288–19,446,838 | 37.7 kb | Distal (>10kb) Multiome | 110 | |
| chr9:19,464,477–19,465,453 | 56.0 kb | Distal (>10kb) Multiome | 427 |
Genomic view of the ACER2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.