Transcription factors with Perturb-seq knockdown data for AACSP1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AACSP1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AACSP1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:178,589,202–178,590,958 | 227.9 kb | Distal (>10kb) Multiome | 378 | |
| chr5:178,626,153–178,627,627 | 191.3 kb | Distal (>10kb) Multiome | 927 | |
| chr5:178,730,157–178,730,973 | 87.7 kb | Distal (>10kb) Multiome | 717 | |
| chr5:178,859,723–178,860,476 | 41.6 kb | Distal (>10kb) Multiome | 813 | |
| chr5:178,895,613–178,896,587 | 77.6 kb | Distal (>10kb) Multiome | 509 | |
| chr5:178,994,469–178,995,315 | 176.4 kb | Distal (>10kb) Multiome | 118 | |
| chr5:179,023,550–179,024,208 | 205.4 kb | Distal (>10kb) Multiome | 632 | |
| chr5:179,060,130–179,060,668 | 241.9 kb | Distal (>10kb) Multiome | 376 |
Genomic view of the AACSP1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.