Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of DNA-templated transcription. Predicted to be located in membrane and nucleus. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for ZNF66. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZNF66 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZNF66, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:20,237,984–20,238,687 | 537.7 kb | Distal (>10kb) Multiome HiCAR | 188 | |
| chr19:20,565,337–20,566,048 | 210.5 kb | Distal (>10kb) Multiome | 386 | |
| chr19:20,660,950–20,661,849 | 114.7 kb | Distal (>10kb) Multiome | 315 | |
| chr19:20,665,001–20,665,677 | 111.0 kb | Distal (>10kb) Multiome | 99 | |
| chr19:20,776,028–20,776,925 | 47 bp | At TSS Multiome | 329 | |
| chr19:20,922,848–20,923,737 | 147.0 kb | Distal (>10kb) Multiome | 286 | |
| chr19:20,999,685–21,000,422 | 223.6 kb | Distal (>10kb) Multiome | 234 | |
| chr19:21,020,242–21,021,293 | 244.4 kb | Distal (>10kb) Multiome | 466 |
Genomic view of the ZNF66 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.