Enables AF-2 domain binding activity; DNA-binding transcription factor binding activity; and transcription corepressor activity. Involved in extracellular negative regulation of signal transduction and negative regulation of transcription by RNA polymerase II. Predicted to be located in extracellular region. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for ZNF653. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZNF653 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZNF653, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:11,243,345–11,243,968 | 261.7 kb | Distal (>10kb) Multiome | 448 | |
| chr19:11,262,060–11,262,653 | 242.7 kb | Distal (>10kb) Multiome | 406 | |
| chr19:11,339,100–11,339,837 | 165.5 kb | Distal (>10kb) Multiome | 506 | |
| chr19:11,346,053–11,346,958 | 158.7 kb | Distal (>10kb) Multiome | 676 | |
| chr19:11,355,078–11,355,735 | 149.8 kb | Distal (>10kb) Multiome | 382 | |
| chr19:11,361,368–11,362,625 | 143.5 kb | Distal (>10kb) Multiome | 256 | |
| chr19:11,374,474–11,375,717 | 130.2 kb | Distal (>10kb) Multiome | 866 | |
| chr19:11,380,761–11,382,262 | 123.8 kb | Distal (>10kb) Multiome | 798 | |
| chr19:11,382,830–11,384,509 | 120.8 kb | Distal (>10kb) Multiome | 761 | |
| chr19:11,418,061–11,419,498 | 86.6 kb | Distal (>10kb) Multiome | 421 | |
| chr19:11,420,614–11,422,979 | 83.0 kb | Distal (>10kb) Multiome | 507 | |
| chr19:11,434,817–11,436,365 | 69.8 kb | Distal (>10kb) Multiome | 927 | |
| chr19:11,479,217–11,480,146 | 25.5 kb | Distal (>10kb) Multiome | 394 | |
| chr19:11,481,847–11,483,040 | 22.5 kb | Distal (>10kb) Multiome | 938 | |
| chr19:11,496,433–11,496,651 | 8.5 kb | Proximal (<10kb) | 235 | |
| chr19:11,504,741–11,506,612 | 177 bp | At TSS Multiome | 854 | |
| chr19:11,528,581–11,529,391 | 23.9 kb | Distal (>10kb) Multiome | 866 | |
| chr19:11,535,847–11,536,353 | 31.0 kb | Distal (>10kb) Multiome | 694 | |
| chr19:11,538,464–11,539,111 | 33.6 kb | Distal (>10kb) Multiome | 235 | |
| chr19:11,539,738–11,540,434 | 34.9 kb | Distal (>10kb) Multiome | 176 | |
| chr19:11,558,908–11,559,777 | 54.1 kb | Distal (>10kb) Multiome | 686 | |
| chr19:11,597,206–11,598,107 | 92.3 kb | Distal (>10kb) Multiome | 914 | |
| chr19:11,639,337–11,640,481 | 134.6 kb | Distal (>10kb) Multiome | 660 | |
| chr19:11,694,497–11,695,341 | 189.8 kb | Distal (>10kb) Multiome | 608 | |
| chr19:11,737,998–11,739,422 | 233.8 kb | Distal (>10kb) Multiome | 704 |
Genomic view of the ZNF653 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.