Transcription factors with Perturb-seq knockdown data for ZFHX4-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZFHX4-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZFHX4-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:76,672,475–76,674,701 | at TSS | At TSS | 486 | |
| chr8:76,676,342–76,676,607 | 3.5 kb | Proximal (<10kb) | 40 | |
| chr8:76,677,304–76,678,556 | 4.5 kb | Proximal (<10kb) | 396 | |
| chr8:76,680,533–76,681,577 | 7.7 kb | Proximal (<10kb) | 458 | |
| chr8:76,681,696–76,683,728 | 8.8 kb | Proximal (<10kb) | 340 |
Genomic view of the ZFHX4-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.