Transcription factors with Perturb-seq knockdown data for ZFAND2A-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZFAND2A-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZFAND2A-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:875,946–877,507 | 283.9 kb | Distal (>10kb) Multiome | 918 | |
| chr7:901,112–902,113 | 258.7 kb | Distal (>10kb) Multiome | 376 | |
| chr7:953,606–955,017 | 205.8 kb | Distal (>10kb) Multiome | 482 | |
| chr7:974,909–975,889 | 184.9 kb | Distal (>10kb) Multiome | 860 | |
| chr7:992,812–993,637 | 167.1 kb | Distal (>10kb) Multiome | 824 | |
| chr7:1,028,063–1,028,589 | 132.1 kb | Distal (>10kb) Multiome | 503 | |
| chr7:1,044,102–1,045,027 | 115.9 kb | Distal (>10kb) Multiome | 715 | |
| chr7:1,059,302–1,060,226 | 100.7 kb | Distal (>10kb) Multiome | 156 | |
| chr7:1,137,933–1,138,672 | 22.1 kb | Distal (>10kb) Multiome | 673 | |
| chr7:1,159,935–1,160,583 | 98 bp | At TSS Multiome | 746 | |
| chr7:1,160,720–1,160,878 | 289 bp | At TSS | 396 | |
| chr7:1,169,907–1,170,159 | 9.5 kb | Proximal (<10kb) | 588 | |
| chr7:1,235,892–1,237,020 | 76.0 kb | Distal (>10kb) Multiome | 184 | |
| chr7:1,368,617–1,369,820 | 208.9 kb | Distal (>10kb) Multiome | 421 | |
| chr7:1,412,524–1,413,381 | 252.6 kb | Distal (>10kb) Multiome | 526 | |
| chr7:1,458,555–1,460,310 | 299.3 kb | Distal (>10kb) Multiome | 677 |
Genomic view of the ZFAND2A-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.