WNK4
WNK lysine deficient protein kinase 4 | PRKWNK4

This gene encodes a member of the WNK family of serine-threonine protein kinases. The kinase is part of the tight junction complex in kidney cells, and regulates the balance between NaCl reabsorption and K(+) secretion. The kinase regulates the activities of several types of ion channels, cotransporters, and exchangers involved in electrolyte flux in epithelial cells. Mutations in this gene result in pseudohypoaldosteronism type IIB.[provided by RefSeq, Sep 2009]

Biological processes 55 terms
ATP binding (GO:0005524)ATP binding (GO:0005524)ERK1 and ERK2 cascade (GO:0070371)aldosterone secretion (GO:0035932)bicellular tight junction (GO:0005923)bicellular tight junction (GO:0005923)bicellular tight junction (GO:0005923)calcium ion homeostasis (GO:0055074)cell body (GO:0044297)cellular response to xenobiotic stimulus (GO:0071466)chloride ion binding (GO:0031404)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytosol (GO:0005829)cytosol (GO:0005829)distal tubule morphogenesis (GO:0072156)gene expression (GO:0010467)homeostatic process (GO:0042592)inflammatory response (GO:0006954)intracellular chloride ion homeostasis (GO:0030644)intracellular signal transduction (GO:0035556)ion channel inhibitor activity (GO:0008200)ion channel inhibitor activity (GO:0008200)macrophage activation (GO:0042116)membrane (GO:0016020)membrane (GO:0016020)monoatomic ion homeostasis (GO:0050801)monoatomic ion homeostasis (GO:0050801)negative regulation of protein localization to plasma membrane (GO:1903077)negative regulation of protein localization to plasma membrane (GO:1903077)negative regulation of sodium ion transport (GO:0010766)negative regulation of sodium ion transport (GO:0010766)potassium ion transmembrane transport (GO:0071805)protein binding (GO:0005515)protein kinase activity (GO:0004672)protein kinase activity (GO:0004672)protein phosphorylation (GO:0006468)protein serine kinase activity (GO:0106310)protein serine/threonine kinase activity (GO:0004674)protein serine/threonine kinase activity (GO:0004674)protein serine/threonine kinase activity (GO:0004674)regulation of blood pressure (GO:0008217)regulation of potassium ion export across plasma membrane (GO:1903764)renal sodium ion absorption (GO:0070294)renal sodium ion absorption (GO:0070294)renal sodium ion absorption (GO:0070294)renal sodium ion transport (GO:0003096)renal sodium ion transport (GO:0003096)response to aldosterone (GO:1904044)response to dietary excess (GO:0002021)response to xenobiotic stimulus (GO:0009410)signal transduction (GO:0007165)signal transduction (GO:0007165)signal transduction (GO:0007165)sodium ion transmembrane transport (GO:0035725)
Expression (TPM)
WNK4 — as a Regulated Gene

TFs regulating WNK4 0 TFs

Transcription factors with Perturb-seq knockdown data for WNK4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = WNK4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to WNK4

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of WNK4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr17:42,773,302–42,773,520 7.1 kb Proximal (<10kb) 875
chr17:42,780,029–42,780,899 at TSS At TSS 712

Genome Browser

Genomic view of the WNK4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr17:42,763,302 – 42,790,899
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq