This gene encodes a member of the WAP-type four-disulfide core (WFDC) domain family. The WFDC domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor. The encoded protein contains four WFDC domains. Most WFDC genes are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the telomeric cluster. Alternatively spliced transcript variants have been observed but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for WFDC3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = WFDC3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of WFDC3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:45,341,603–45,342,760 | 449.6 kb | Distal (>10kb) Multiome HiCAR | 696 | |
| chr20:45,343,396–45,344,073 | 448.2 kb | Distal (>10kb) Multiome HiCAR | 485 | |
| chr20:45,347,673–45,348,799 | 443.5 kb | Distal (>10kb) Multiome HiCAR | 805 | |
| chr20:45,509,025–45,509,824 | 282.5 kb | Distal (>10kb) Multiome | 109 | |
| chr20:45,764,655–45,765,666 | 26.8 kb | Distal (>10kb) Multiome | 192 | |
| chr20:45,770,709–45,771,657 | 20.7 kb | Distal (>10kb) Multiome | 436 | |
| chr20:45,791,633–45,792,259 | 11 bp | At TSS Multiome | 882 | |
| chr20:45,812,087–45,813,052 | 20.5 kb | Distal (>10kb) Multiome | 695 | |
| chr20:45,833,493–45,834,622 | 42.0 kb | Distal (>10kb) Multiome | 833 | |
| chr20:45,856,871–45,858,137 | 65.6 kb | Distal (>10kb) Multiome | 884 | |
| chr20:45,880,796–45,881,524 | 89.2 kb | Distal (>10kb) Multiome | 738 | |
| chr20:45,890,127–45,891,800 | 99.4 kb | Distal (>10kb) Multiome | 1095 | |
| chr20:45,910,538–45,912,397 | 120.3 kb | Distal (>10kb) Multiome | 713 | |
| chr20:45,933,591–45,935,780 | 143.2 kb | Distal (>10kb) Multiome | 1195 | |
| chr20:45,971,553–45,972,790 | 180.2 kb | Distal (>10kb) Multiome | 1206 | |
| chr20:46,004,647–46,005,315 | 213.0 kb | Distal (>10kb) Multiome | 207 | |
| chr20:46,011,604–46,012,166 | 220.0 kb | Distal (>10kb) Multiome | 239 | |
| chr20:46,013,565–46,014,586 | 222.2 kb | Distal (>10kb) Multiome | 735 | |
| chr20:46,021,209–46,023,166 | 230.1 kb | Distal (>10kb) Multiome | 679 | |
| chr20:46,089,092–46,090,355 | 297.9 kb | Distal (>10kb) Multiome | 1047 |
Genomic view of the WFDC3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.