Transcription factors with Perturb-seq knockdown data for WDFY3-AS2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = WDFY3-AS2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of WDFY3-AS2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:84,870,270–84,871,015 | 96.1 kb | Distal (>10kb) Multiome | 271 | |
| chr4:84,965,521–84,967,570 | 25 bp | At TSS Multiome | 838 | |
| chr4:85,127,090–85,129,185 | 161.0 kb | Distal (>10kb) Multiome | 436 |
Genomic view of the WDFY3-AS2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.