Transcription factors with Perturb-seq knockdown data for WASL-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = WASL-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of WASL-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:123,533,937–123,535,843 | 214.2 kb | Distal (>10kb) Multiome | 560 | |
| chr7:123,557,224–123,558,428 | 191.2 kb | Distal (>10kb) Multiome | 732 | |
| chr7:123,594,565–123,595,316 | 154.1 kb | Distal (>10kb) Multiome | 183 | |
| chr7:123,637,247–123,638,386 | 111.1 kb | Distal (>10kb) Multiome | 95 | |
| chr7:123,657,889–123,659,811 | 90.6 kb | Distal (>10kb) Multiome | 165 | |
| chr7:123,692,360–123,692,863 | 56.4 kb | Distal (>10kb) Multiome | 179 | |
| chr7:123,747,604–123,749,779 | 77 bp | At TSS Multiome | 1065 | |
| chr7:124,031,863–124,034,280 | 283.7 kb | Distal (>10kb) Multiome | 500 |
Genomic view of the WASL-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.