This gene encodes a ubiquitin specific processing protease that was first identified as a substrate of the VHL (von Hippel-Lindau disease) protein E3 ubiquitin ligase complex. In addition to being ubiquitinated by the VHL-E3 ligase complex, this enzyme deubiquitinates hypoxia-inducible factor (HIF)-1 alpha and thereby causes increased expression of HIF-1alpha targeted genes which play a role in angiogenesis, glucose metabolism, cell proliferation and metastasis. The enzyme encoded by this gene also regulates G-protein coupled receptor signaling by mediating the deubiquitination of beta-2 adrenergic receptor (ADRB2). This enzyme is a ubiquitously expressed thiolester hydrolase. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jan 2013]
Transcription factors with Perturb-seq knockdown data for USP20. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = USP20 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of USP20, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:129,557,196–129,557,935 | 277.9 kb | Distal (>10kb) Multiome | 718 | |
| chr9:129,596,686–129,597,656 | 238.4 kb | Distal (>10kb) Multiome | 700 | |
| chr9:129,610,548–129,611,334 | 224.6 kb | Distal (>10kb) Multiome | 630 | |
| chr9:129,626,055–129,626,580 | 209.2 kb | Distal (>10kb) Multiome | 825 | |
| chr9:129,641,895–129,642,673 | 193.2 kb | Distal (>10kb) Multiome | 919 | |
| chr9:129,802,988–129,803,555 | 32.3 kb | Distal (>10kb) Multiome | 727 | |
| chr9:129,835,192–129,835,687 | 88 bp | At TSS Multiome | 701 | |
| chr9:130,042,478–130,043,723 | 207.8 kb | Distal (>10kb) Multiome | 793 |
Genomic view of the USP20 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.