Transcription factors with Perturb-seq knockdown data for TWSG1-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TWSG1-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TWSG1-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr18:9,102,098–9,103,261 | 231.8 kb | Distal (>10kb) Multiome | 815 | |
| chr18:9,135,781–9,137,908 | 197.0 kb | Distal (>10kb) Multiome | 1018 | |
| chr18:9,264,819–9,265,280 | 69.3 kb | Distal (>10kb) Multiome | 49 | |
| chr18:9,333,728–9,335,352 | 197 bp | At TSS Multiome | 738 | |
| chr18:9,474,512–9,476,246 | 140.9 kb | Distal (>10kb) Multiome | 971 | |
| chr18:9,613,721–9,615,403 | 280.5 kb | Distal (>10kb) Multiome | 740 |
Genomic view of the TWSG1-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.