Transcription factors with Perturb-seq knockdown data for TWF2-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TWF2-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TWF2-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:52,231,607–52,231,792 | 7.5 kb | Proximal (<10kb) | 286 | |
| chr3:52,237,653–52,238,416 | 838 bp | At TSS | 535 | |
| chr3:52,238,767–52,239,716 | at TSS | At TSS | 763 | |
| chr3:52,245,446–52,246,450 | 6.2 kb | Proximal (<10kb) | 553 |
Genomic view of the TWF2-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.