This gene encodes a component of the gamma-tubulin ring complex, which is required for microtubule nucleation. In mammalian cells, the protein localizes to centrosomes in association with gamma-tubulin. Crystal structure analysis revealed a structure composed of five helical bundles arranged around conserved hydrophobic cores. An exposed surface area located in the C-terminal domain is essential and sufficient for direct binding to gamma-tubulin. Mutations in this gene that alter microtubule organization are associated with microcephaly and chorioretinopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]
Transcription factors with Perturb-seq knockdown data for TUBGCP4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TUBGCP4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TUBGCP4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr15:43,184,963–43,185,969 | 185.7 kb | Distal (>10kb) Multiome | 807 | |
| chr15:43,330,217–43,330,762 | 40.6 kb | Distal (>10kb) Multiome | 845 | |
| chr15:43,370,765–43,371,726 | 228 bp | At TSS Multiome | 774 | |
| chr15:43,492,673–43,493,432 | 122.1 kb | Distal (>10kb) Multiome | 544 | |
| chr15:43,510,550–43,511,326 | 139.8 kb | Distal (>10kb) Multiome | 683 |
Genomic view of the TUBGCP4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.