Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for TRMT12. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TRMT12 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TRMT12, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:124,371,408–124,373,569 | 78.2 kb | Distal (>10kb) Multiome | 891 | |
| chr8:124,450,261–124,450,521 | 394 bp | At TSS | 370 | |
| chr8:124,450,699–124,451,029 | at TSS | At TSS | 556 | |
| chr8:124,473,813–124,475,636 | 23.9 kb | Distal (>10kb) Multiome | 970 | |
| chr8:124,538,847–124,539,474 | 88.2 kb | Distal (>10kb) Multiome | 921 | |
| chr8:124,726,992–124,728,984 | 277.7 kb | Distal (>10kb) Multiome | 609 |
Genomic view of the TRMT12 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.