This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for TOPBP1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TOPBP1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TOPBP1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:132,721,513–132,723,554 | 939.2 kb | Distal (>10kb) Multiome HiCAR | 756 | |
| chr3:133,384,300–133,384,776 | 277.3 kb | Distal (>10kb) Multiome | 247 | |
| chr3:133,661,440–133,662,508 | 27 bp | At TSS Multiome | 728 | |
| chr3:133,663,657–133,664,042 | 1.7 kb | Proximal (<10kb) | 200 | |
| chr3:133,805,616–133,806,520 | 143.9 kb | Distal (>10kb) Multiome | 710 | |
| chr3:133,894,751–133,896,433 | 233.8 kb | Distal (>10kb) Multiome | 424 | |
| chr3:133,927,020–133,928,082 | 265.7 kb | Distal (>10kb) Multiome | 363 |
Genomic view of the TOPBP1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.