This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013]
Transcription factors with Perturb-seq knockdown data for TOP3B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TOP3B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TOP3B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:21,735,345–21,736,601 | 246.8 kb | Distal (>10kb) Multiome | 774 | |
| chr22:21,864,937–21,865,391 | 117.7 kb | Distal (>10kb) Multiome | 184 | |
| chr22:21,866,936–21,868,402 | 114.9 kb | Distal (>10kb) Multiome | 929 | |
| chr22:21,925,450–21,926,079 | 57.0 kb | Distal (>10kb) Multiome | 169 | |
| chr22:21,938,040–21,938,786 | 44.5 kb | Distal (>10kb) Multiome | 871 | |
| chr22:21,951,964–21,953,251 | 29.9 kb | Distal (>10kb) Multiome | 645 | |
| chr22:21,982,701–21,982,944 | at TSS | At TSS | 959 |
Genomic view of the TOP3B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.