TNFSF4
TNF superfamily member 4 | CD252, OX-40L, gp34, TXGP1

This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Biological processes 55 terms
T-helper 2 cell activation (GO:0035712)acute inflammatory response (GO:0002526)cell communication (GO:0007154)cell surface (GO:0009986)cellular response to lipopolysaccharide (GO:0071222)cellular response to prostaglandin E stimulus (GO:0071380)cytokine activity (GO:0005125)defense response to nematode (GO:0002215)extracellular region (GO:0005576)extracellular region (GO:0005576)immune response (GO:0006955)inflammatory response (GO:0006954)membrane (GO:0016020)memory T cell activation (GO:0035709)negative regulation of T-helper 1 cell differentiation (GO:0045626)negative regulation of interleukin-17 production (GO:0032700)negative regulation of regulatory T cell differentiation (GO:0045590)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of type II interferon production (GO:0032689)plasma membrane (GO:0005886)plasma membrane (GO:0005886)positive regulation of B cell activation (GO:0050871)positive regulation of CD4-positive, alpha-beta T cell costimulation (GO:1900281)positive regulation of CD4-positive, alpha-beta T cell differentiation (GO:0043372)positive regulation of T cell cytokine production (GO:0002726)positive regulation of T cell proliferation (GO:0042102)positive regulation of T-helper 2 cell differentiation (GO:0045630)positive regulation of alpha-beta T cell proliferation (GO:0046641)positive regulation of cell population proliferation (GO:0008284)positive regulation of chemokine production (GO:0032722)positive regulation of cytokine production (GO:0001819)positive regulation of immunoglobulin mediated immune response (GO:0002891)positive regulation of immunoglobulin production (GO:0002639)positive regulation of inflammatory response (GO:0050729)positive regulation of interleukin-10 production (GO:0032733)positive regulation of interleukin-12 production (GO:0032735)positive regulation of interleukin-13 production (GO:0032736)positive regulation of interleukin-4 production (GO:0032753)positive regulation of interleukin-4-dependent isotype switching to IgE isotypes (GO:2000572)positive regulation of interleukin-6 production (GO:0032755)positive regulation of memory T cell differentiation (GO:0043382)positive regulation of type 2 immune response (GO:0002830)positive regulation of type II interferon production (GO:0032729)protein binding (GO:0005515)regulation of adaptive immune response (GO:0002819)regulation of inflammatory response (GO:0050727)response to nitrogen dioxide (GO:0035713)response to virus (GO:0009615)signal transduction (GO:0007165)signaling (GO:0023052)signaling receptor binding (GO:0005102)signaling receptor binding (GO:0005102)tumor necrosis factor receptor binding (GO:0005164)tumor necrosis factor receptor superfamily binding (GO:0032813)tumor necrosis factor receptor superfamily binding (GO:0032813)
Expression (TPM)
TNFSF4 — as a Regulated Gene

TFs regulating TNFSF4 0 TFs

Transcription factors with Perturb-seq knockdown data for TNFSF4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TNFSF4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to TNFSF4

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TNFSF4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr1:173,474,492–173,475,513 1.5 kb Proximal (<10kb) 348
chr1:173,476,153–173,478,392 at TSS At TSS 991

Genome Browser

Genomic view of the TNFSF4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr1:173,464,492 – 173,488,392
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq