Transcription factors with Perturb-seq knockdown data for TMED8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TMED8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TMED8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:77,085,220–77,085,934 | 291.5 kb | Distal (>10kb) Multiome | 132 | |
| chr14:77,088,297–77,088,792 | 288.6 kb | Distal (>10kb) Multiome | 234 | |
| chr14:77,097,312–77,099,236 | 279.0 kb | Distal (>10kb) Multiome | 948 | |
| chr14:77,124,505–77,125,759 | 252.1 kb | Distal (>10kb) Multiome | 289 | |
| chr14:77,139,459–77,142,559 | 237.3 kb | Distal (>10kb) Multiome | 507 | |
| chr14:77,181,185–77,182,207 | 195.4 kb | Distal (>10kb) Multiome | 485 | |
| chr14:77,270,588–77,271,586 | 106.0 kb | Distal (>10kb) Multiome | 147 | |
| chr14:77,320,198–77,322,121 | 55.9 kb | Distal (>10kb) Multiome | 915 | |
| chr14:77,376,423–77,377,683 | 37 bp | At TSS Multiome | 630 | |
| chr14:77,456,961–77,458,831 | 80.6 kb | Distal (>10kb) Multiome | 878 | |
| chr14:77,498,012–77,499,509 | 121.8 kb | Distal (>10kb) Multiome | 599 | |
| chr14:77,615,862–77,617,380 | 239.8 kb | Distal (>10kb) Multiome | 892 | |
| chr14:77,654,873–77,655,664 | 278.1 kb | Distal (>10kb) Multiome | 287 |
Genomic view of the TMED8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.