Transcription factors with Perturb-seq knockdown data for TMED2-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TMED2-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TMED2-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:123,363,804–123,365,980 | 220.0 kb | Distal (>10kb) Multiome | 1015 | |
| chr12:123,383,367–123,384,963 | 201.2 kb | Distal (>10kb) Multiome | 836 | |
| chr12:123,388,500–123,389,409 | 196.2 kb | Distal (>10kb) Multiome | 446 | |
| chr12:123,436,072–123,436,895 | 148.5 kb | Distal (>10kb) Multiome | 742 | |
| chr12:123,458,045–123,458,697 | 126.7 kb | Distal (>10kb) Multiome | 925 | |
| chr12:123,533,444–123,534,540 | 51.2 kb | Distal (>10kb) Multiome | 718 | |
| chr12:123,583,890–123,585,003 | 623 bp | At TSS Multiome | 928 | |
| chr12:123,601,617–123,602,401 | 16.9 kb | Distal (>10kb) Multiome | 1076 | |
| chr12:123,633,273–123,633,970 | 48.6 kb | Distal (>10kb) Multiome | 886 | |
| chr12:123,670,899–123,671,414 | 86.0 kb | Distal (>10kb) Multiome | 469 | |
| chr12:123,711,684–123,712,955 | 127.2 kb | Distal (>10kb) Multiome | 771 | |
| chr12:123,761,832–123,763,058 | 177.0 kb | Distal (>10kb) Multiome | 416 |
Genomic view of the TMED2-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.