The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1. [provided by RefSeq, May 2012]
Transcription factors with Perturb-seq knockdown data for TJP3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TJP3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TJP3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:3,434,837–3,435,636 | 286.5 kb | Distal (>10kb) Multiome | 777 | |
| chr19:3,444,085–3,444,710 | 277.2 kb | Distal (>10kb) Multiome | 150 | |
| chr19:3,500,403–3,501,074 | 220.9 kb | Distal (>10kb) Multiome | 886 | |
| chr19:3,505,575–3,507,061 | 215.6 kb | Distal (>10kb) Multiome | 838 | |
| chr19:3,557,019–3,558,238 | 164.1 kb | Distal (>10kb) Multiome | 811 | |
| chr19:3,572,147–3,574,664 | 147.6 kb | Distal (>10kb) Multiome | 737 | |
| chr19:3,576,363–3,578,543 | 144.6 kb | Distal (>10kb) Multiome | 346 | |
| chr19:3,606,342–3,607,177 | 114.9 kb | Distal (>10kb) Multiome | 377 | |
| chr19:3,626,518–3,627,304 | 94.7 kb | Distal (>10kb) Multiome | 833 | |
| chr19:3,699,968–3,700,899 | 21.2 kb | Distal (>10kb) Multiome | 489 | |
| chr19:3,721,462–3,721,997 | 99 bp | At TSS Multiome | 760 | |
| chr19:3,722,652–3,723,093 | 1.2 kb | Proximal (<10kb) Multiome | 106 | |
| chr19:3,761,103–3,762,150 | 39.9 kb | Distal (>10kb) Multiome | 839 | |
| chr19:3,762,300–3,763,344 | 41.2 kb | Distal (>10kb) Multiome | 817 | |
| chr19:3,868,451–3,869,181 | 147.4 kb | Distal (>10kb) Multiome | 233 | |
| chr19:3,932,937–3,933,664 | 211.5 kb | Distal (>10kb) Multiome | 198 | |
| chr19:3,968,623–3,969,280 | 247.1 kb | Distal (>10kb) Multiome | 547 | |
| chr19:3,970,535–3,971,713 | 249.5 kb | Distal (>10kb) Multiome | 728 | |
| chr19:3,985,032–3,985,862 | 263.7 kb | Distal (>10kb) Multiome | 932 | |
| chr19:4,006,992–4,008,553 | 285.8 kb | Distal (>10kb) Multiome | 888 |
Genomic view of the TJP3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.