This gene encodes a threonine synthase-like protein. A similar enzyme in mouse can catalyze the degradation of O-phospho-homoserine to a-ketobutyrate, phosphate, and ammonia. This protein also has phospho-lyase activity on both gamma and beta phosphorylated substrates. In mouse an alternatively spliced form of this protein has been shown to act as a cytokine and can induce the production of the inflammatory cytokine IL6 in osteoblasts. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
Transcription factors with Perturb-seq knockdown data for THNSL2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = THNSL2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of THNSL2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:88,015,193–88,017,502 | 153.5 kb | Distal (>10kb) Multiome | 1123 | |
| chr2:88,055,077–88,056,028 | 114.5 kb | Distal (>10kb) Multiome | 869 | |
| chr2:88,159,977–88,160,386 | 10.0 kb | Proximal (<10kb) | 280 | |
| chr2:88,170,010–88,170,891 | 50 bp | At TSS Multiome | 211 | |
| chr2:88,254,337–88,255,161 | 84.4 kb | Distal (>10kb) Multiome | 121 | |
| chr2:88,329,732–88,330,828 | 160.0 kb | Distal (>10kb) Multiome | 408 | |
| chr2:88,350,256–88,351,496 | 180.7 kb | Distal (>10kb) Multiome | 374 | |
| chr2:88,415,840–88,416,802 | 245.9 kb | Distal (>10kb) Multiome | 135 | |
| chr2:88,451,554–88,453,275 | 282.2 kb | Distal (>10kb) Multiome | 267 |
Genomic view of the THNSL2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.