Transcription factors with Perturb-seq knockdown data for TESHL. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TESHL upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TESHL, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:216,687,737–216,687,960 | 6.6 kb | Proximal (<10kb) | 89 | |
| chr2:216,691,972–216,693,060 | 1.5 kb | Proximal (<10kb) | 383 | |
| chr2:216,693,166–216,693,613 | 914 bp | At TSS | 180 | |
| chr2:216,694,105–216,695,084 | at TSS | At TSS | 316 | |
| chr2:216,695,439–216,696,027 | 911 bp | At TSS | 258 | |
| chr2:216,696,754–216,696,956 | 2.2 kb | Proximal (<10kb) | 84 | |
| chr2:216,698,881–216,699,135 | 4.4 kb | Proximal (<10kb) | 164 |
Genomic view of the TESHL locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.