Transcription factors with Perturb-seq knockdown data for STX17-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = STX17-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of STX17-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:99,819,265–99,820,671 | 86.6 kb | Distal (>10kb) Multiome | 934 | |
| chr9:99,821,209–99,823,110 | 84.9 kb | Distal (>10kb) Multiome | 759 | |
| chr9:99,823,697–99,825,101 | 81.8 kb | Distal (>10kb) Multiome | 396 | |
| chr9:99,828,488–99,829,180 | 77.8 kb | Distal (>10kb) Multiome | 184 | |
| chr9:99,906,186–99,907,530 | 180 bp | At TSS Multiome | 862 | |
| chr9:100,098,013–100,099,995 | 192.6 kb | Distal (>10kb) Multiome | 884 |
Genomic view of the STX17-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.