OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009 [PubMed 19854130]).[supplied by OMIM, Nov 2009]
Transcription factors with Perturb-seq knockdown data for STN1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = STN1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of STN1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:103,661,427–103,661,981 | 256.5 kb | Distal (>10kb) Multiome | 301 | |
| chr10:103,692,163–103,693,782 | 225.8 kb | Distal (>10kb) Multiome | 288 | |
| chr10:103,771,858–103,772,878 | 145.7 kb | Distal (>10kb) Multiome | 532 | |
| chr10:103,829,792–103,830,463 | 88.0 kb | Distal (>10kb) Multiome | 65 | |
| chr10:103,836,976–103,837,450 | 81.0 kb | Distal (>10kb) Multiome | 52 | |
| chr10:103,839,572–103,841,382 | 77.8 kb | Distal (>10kb) Multiome | 306 | |
| chr10:103,854,330–103,856,292 | 62.5 kb | Distal (>10kb) Multiome | 649 | |
| chr10:103,908,255–103,908,617 | 9.6 kb | Proximal (<10kb) | 704 | |
| chr10:103,917,536–103,919,139 | 159 bp | At TSS Multiome | 926 | |
| chr10:103,921,803–103,921,995 | 3.6 kb | Proximal (<10kb) | 199 | |
| chr10:103,966,618–103,967,712 | 49.0 kb | Distal (>10kb) Multiome | 758 | |
| chr10:104,045,227–104,046,125 | 127.4 kb | Distal (>10kb) Multiome | 116 | |
| chr10:104,057,535–104,058,241 | 139.7 kb | Distal (>10kb) Multiome | 66 | |
| chr10:104,120,766–104,122,782 | 203.8 kb | Distal (>10kb) Multiome | 959 | |
| chr10:104,155,284–104,156,268 | 237.7 kb | Distal (>10kb) Multiome | 175 | |
| chr10:104,254,370–104,255,379 | 336.7 kb | Distal (>10kb) Multiome HiCAR | 714 |
Genomic view of the STN1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.