Transcription factors with Perturb-seq knockdown data for ST8SIA5-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ST8SIA5-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ST8SIA5-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr18:46,466,054–46,466,835 | 290.3 kb | Distal (>10kb) Multiome | 48 | |
| chr18:46,657,856–46,658,572 | 98.6 kb | Distal (>10kb) Multiome | 342 | |
| chr18:46,755,115–46,755,298 | 1.6 kb | Proximal (<10kb) | 37 | |
| chr18:46,756,207–46,757,254 | 23 bp | At TSS Multiome | 210 | |
| chr18:46,916,768–46,918,244 | 160.7 kb | Distal (>10kb) Multiome | 715 | |
| chr18:46,946,455–46,947,310 | 189.9 kb | Distal (>10kb) Multiome | 249 |
Genomic view of the ST8SIA5-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.