The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein prefers glycoproteins rather than glycolipids as substrates and shows restricted substrate specificity, utilizing only the trisaccharide sequence Neu5Ac-alpha-2,3-Gal-beta-1,3-GalNAc. In addition, it is involved in the synthesis of ganglioside GD1A from GM1B. The encoded protein is normally found in the Golgi apparatus but can be proteolytically processed to a soluble form. This protein is a member of glycosyltransferase family 29. Transcript variants encoding different isoforms have been found for this gene. Readthrough transcripts exist for this gene and the downstream ST6GALNAC6 gene. [provided by RefSeq, Jan 2022]
Transcription factors with Perturb-seq knockdown data for ST6GALNAC4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ST6GALNAC4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ST6GALNAC4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:127,699,155–127,699,632 | 217.6 kb | Distal (>10kb) Multiome | 440 | |
| chr9:127,715,013–127,716,345 | 201.2 kb | Distal (>10kb) Multiome | 735 | |
| chr9:127,734,444–127,735,606 | 181.8 kb | Distal (>10kb) Multiome | 836 | |
| chr9:127,741,739–127,743,092 | 175.0 kb | Distal (>10kb) Multiome HiCAR | 367 | |
| chr9:127,754,123–127,755,506 | 162.7 kb | Distal (>10kb) Multiome | 279 | |
| chr9:127,770,807–127,771,925 | 145.5 kb | Distal (>10kb) Multiome | 550 | |
| chr9:127,784,682–127,787,009 | 130.5 kb | Distal (>10kb) Multiome | 858 | |
| chr9:127,802,385–127,803,632 | 114.1 kb | Distal (>10kb) Multiome HiCAR | 867 | |
| chr9:127,809,338–127,810,272 | 107.2 kb | Distal (>10kb) Multiome HiCAR | 276 | |
| chr9:127,825,222–127,826,046 | 91.4 kb | Distal (>10kb) Multiome | 451 | |
| chr9:127,828,234–127,829,024 | 88.4 kb | Distal (>10kb) Multiome | 521 | |
| chr9:127,897,316–127,897,796 | 19.5 kb | Distal (>10kb) Multiome | 437 | |
| chr9:127,898,861–127,900,094 | 17.5 kb | Distal (>10kb) Multiome | 630 | |
| chr9:127,916,386–127,917,505 | 92 bp | At TSS Multiome | 474 | |
| chr9:127,921,626–127,921,895 | 4.6 kb | Proximal (<10kb) | 375 | |
| chr9:127,926,756–127,928,574 | 10.5 kb | Distal (>10kb) Multiome | 411 | |
| chr9:127,930,321–127,931,068 | 13.7 kb | Distal (>10kb) Multiome | 537 | |
| chr9:127,937,578–127,938,244 | 20.9 kb | Distal (>10kb) Multiome | 674 | |
| chr9:127,980,172–127,981,966 | 64.1 kb | Distal (>10kb) Multiome | 691 | |
| chr9:128,034,940–128,035,527 | 118.3 kb | Distal (>10kb) Multiome | 480 | |
| chr9:128,066,577–128,068,774 | 151.0 kb | Distal (>10kb) Multiome | 874 | |
| chr9:128,097,872–128,098,910 | 181.4 kb | Distal (>10kb) Multiome | 712 | |
| chr9:128,117,658–128,118,328 | 200.8 kb | Distal (>10kb) Multiome | 640 | |
| chr9:128,127,314–128,128,793 | 211.3 kb | Distal (>10kb) Multiome | 899 | |
| chr9:128,159,371–128,160,928 | 243.1 kb | Distal (>10kb) Multiome | 824 | |
| chr9:128,191,300–128,192,050 | 274.7 kb | Distal (>10kb) Multiome | 865 | |
| chr9:128,203,004–128,204,704 | 287.3 kb | Distal (>10kb) Multiome | 690 |
Genomic view of the ST6GALNAC4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.