Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. This gene is one member of a family of beta-spectrin genes. The encoded protein localizes to the nuclear matrix, PML nuclear bodies, and cytoplasmic vesicles. A highly similar gene in the mouse is required for localization of specific membrane proteins in polarized regions of neurons. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for SPTBN4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SPTBN4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SPTBN4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:40,464,424–40,464,609 | 1.6 kb | Proximal (<10kb) | 444 | |
| chr19:40,465,326–40,466,635 | at TSS | At TSS | 815 | |
| chr19:40,523,350–40,523,568 | 7.2 kb | Proximal (<10kb) | 505 | |
| chr19:40,528,978–40,529,879 | 885 bp | At TSS | 601 | |
| chr19:40,529,987–40,530,863 | at TSS | At TSS | 384 | |
| chr19:40,570,181–40,571,253 | at TSS | At TSS | 598 | |
| chr19:40,576,133–40,577,495 | 5.1 kb | Proximal (<10kb) | 616 | |
| chr19:40,578,500–40,578,793 | 7.5 kb | Proximal (<10kb) | 227 |
Genomic view of the SPTBN4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.