Enables ubiquitin-like ligase-substrate adaptor activity. Involved in several processes, including negative regulation of cGAS/STING signaling pathway; negative regulation of epithelial to mesenchymal transition; and protein K48-linked ubiquitination. Part of Cul5-RING ubiquitin ligase complex. Is active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for SPSB3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SPSB3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SPSB3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:1,492,797–1,493,962 | 289.2 kb | Distal (>10kb) Multiome | 710 | |
| chr16:1,610,035–1,611,264 | 171.9 kb | Distal (>10kb) Multiome | 745 | |
| chr16:1,611,870–1,612,834 | 170.3 kb | Distal (>10kb) Multiome | 718 | |
| chr16:1,677,899–1,679,024 | 104.4 kb | Distal (>10kb) Multiome | 709 | |
| chr16:1,705,380–1,706,702 | 76.4 kb | Distal (>10kb) Multiome | 933 | |
| chr16:1,763,511–1,764,353 | 18.5 kb | Distal (>10kb) Multiome | 261 | |
| chr16:1,770,847–1,771,973 | 11.0 kb | Distal (>10kb) Multiome | 485 | |
| chr16:1,772,565–1,774,049 | 9.2 kb | Proximal (<10kb) Multiome | 956 | |
| chr16:1,781,749–1,783,420 | 209 bp | At TSS Multiome | 761 | |
| chr16:1,826,102–1,827,535 | 44.4 kb | Distal (>10kb) Multiome | 1006 | |
| chr16:1,918,171–1,918,773 | 135.9 kb | Distal (>10kb) Multiome | 129 | |
| chr16:1,929,283–1,930,271 | 147.3 kb | Distal (>10kb) Multiome | 780 | |
| chr16:1,942,671–1,943,761 | 160.8 kb | Distal (>10kb) Multiome | 750 | |
| chr16:1,958,923–1,960,140 | 176.9 kb | Distal (>10kb) Multiome | 826 | |
| chr16:1,964,300–1,965,434 | 182.4 kb | Distal (>10kb) Multiome | 933 | |
| chr16:1,971,481–1,972,435 | 189.4 kb | Distal (>10kb) Multiome | 1010 | |
| chr16:1,979,111–1,980,324 | 196.9 kb | Distal (>10kb) Multiome | 296 | |
| chr16:1,983,450–1,984,851 | 201.4 kb | Distal (>10kb) Multiome | 917 | |
| chr16:1,989,307–1,993,568 | 209.8 kb | Distal (>10kb) Multiome | 706 | |
| chr16:2,003,882–2,004,348 | 221.5 kb | Distal (>10kb) Multiome | 619 | |
| chr16:2,009,742–2,010,504 | 227.3 kb | Distal (>10kb) Multiome | 752 | |
| chr16:2,022,700–2,023,923 | 240.7 kb | Distal (>10kb) Multiome | 682 | |
| chr16:2,026,629–2,027,149 | 244.3 kb | Distal (>10kb) Multiome | 249 | |
| chr16:2,047,366–2,048,252 | 265.3 kb | Distal (>10kb) Multiome | 875 |
Genomic view of the SPSB3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.