SPOUT1
SPOUT domain containing methyltransferase 1 | CENP-32, HSPC109, C9orf114

Enables miRNA binding activity. Involved in maintenance of centrosome location and miRNA processing. Acts upstream of with a positive effect on post-transcriptional regulation of gene expression. Located in kinetochore; mitotic spindle; and spindle pole centrosome. [provided by Alliance of Genome Resources, Apr 2025]

Biological processes 25 terms
RNA binding (GO:0003723)RNA methyltransferase activity (GO:0008173)RNA methyltransferase activity (GO:0008173)RNA processing (GO:0006396)S-adenosyl-L-methionine binding (GO:1904047)S-adenosylmethionine-dependent methyltransferase activity (GO:0008757)centrosome (GO:0005813)kinetochore (GO:0000776)kinetochore (GO:0000776)maintenance of centrosome location (GO:0051661)maintenance of centrosome location (GO:0051661)methyltransferase activity (GO:0008168)miRNA binding (GO:0035198)miRNA binding (GO:0035198)miRNA processing (GO:0035196)miRNA processing (GO:0035196)mitotic spindle (GO:0072686)mitotic spindle (GO:0072686)post-transcriptional regulation of gene expression (GO:0010608)protein binding (GO:0005515)rRNA (uridine-N3-)-methyltransferase activity (GO:0070042)rRNA base methylation (GO:0070475)spindle (GO:0005819)spindle pole centrosome (GO:0031616)spindle pole centrosome (GO:0031616)
Expression (TPM)
SPOUT1 — as a Regulated Gene

TFs regulating SPOUT1 0 TFs

Transcription factors with Perturb-seq knockdown data for SPOUT1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SPOUT1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to SPOUT1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SPOUT1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr9:128,552,006–128,553,125 277.3 kb Distal (>10kb) Multiome 839
chr9:128,656,436–128,657,126 173.0 kb Distal (>10kb) Multiome 1009
chr9:128,683,393–128,684,010 146.2 kb Distal (>10kb) Multiome 296
chr9:128,684,170–128,684,813 145.1 kb Distal (>10kb) Multiome 574
chr9:128,688,449–128,689,713 140.7 kb Distal (>10kb) Multiome 805
chr9:128,702,246–128,702,946 127.2 kb Distal (>10kb) Multiome 720
chr9:128,723,691–128,725,229 105.6 kb Distal (>10kb) Multiome 821
chr9:128,771,658–128,772,301 57.8 kb Distal (>10kb) Multiome 940
chr9:128,786,928–128,787,516 42.6 kb Distal (>10kb) Multiome 1040
chr9:128,818,208–128,819,406 11.3 kb Distal (>10kb) Multiome 839
chr9:128,829,505–128,830,189 43 bp At TSS Multiome 782
chr9:128,881,863–128,882,454 52.3 kb Distal (>10kb) Multiome 777
chr9:128,921,941–128,922,727 92.5 kb Distal (>10kb) Multiome 378
chr9:128,947,329–128,947,815 117.8 kb Distal (>10kb) Multiome 865
chr9:129,027,821–129,028,677 198.6 kb Distal (>10kb) Multiome 639
chr9:129,036,276–129,036,937 206.7 kb Distal (>10kb) Multiome 736
chr9:129,080,488–129,081,502 251.2 kb Distal (>10kb) Multiome 755
chr9:129,110,314–129,111,708 281.3 kb Distal (>10kb) Multiome 881

Genome Browser

Genomic view of the SPOUT1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr9:128,542,006 – 129,121,708
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq
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