Involved in sperm axoneme assembly. Located in cytosol; nuclear body; and sperm flagellum. Implicated in spermatogenic failure 43. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for SPEF2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SPEF2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SPEF2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:35,229,175–35,230,682 | 388.3 kb | Distal (>10kb) Multiome HiCAR | 585 | |
| chr5:35,317,354–35,318,124 | 300.1 kb | Distal (>10kb) Multiome | 100 | |
| chr5:35,337,306–35,338,334 | 280.0 kb | Distal (>10kb) Multiome | 120 | |
| chr5:35,600,451–35,601,459 | 17.0 kb | Distal (>10kb) Multiome | 72 | |
| chr5:35,617,381–35,618,518 | 7 bp | At TSS Multiome | 757 |
Genomic view of the SPEF2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.