This gene encodes a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. A retrotransposed pseudogene is present on chromosome 10q22.[provided by RefSeq, Jan 2009]
Transcription factors with Perturb-seq knockdown data for SPA17. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SPA17 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SPA17, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:124,622,373–124,623,302 | 51.1 kb | Distal (>10kb) Multiome | 828 | |
| chr11:124,669,221–124,669,424 | 4.5 kb | Proximal (<10kb) | 202 | |
| chr11:124,673,250–124,674,391 | 95 bp | At TSS Multiome | 715 | |
| chr11:124,739,482–124,740,822 | 66.0 kb | Distal (>10kb) Multiome | 498 | |
| chr11:124,745,504–124,747,699 | 73.0 kb | Distal (>10kb) Multiome | 676 | |
| chr11:124,758,225–124,760,203 | 84.7 kb | Distal (>10kb) Multiome | 594 | |
| chr11:124,799,320–124,800,957 | 126.6 kb | Distal (>10kb) Multiome | 893 | |
| chr11:124,839,093–124,840,110 | 165.9 kb | Distal (>10kb) Multiome HiCAR | 246 | |
| chr11:124,842,870–124,844,108 | 169.5 kb | Distal (>10kb) Multiome | 219 | |
| chr11:124,875,886–124,877,815 | 202.6 kb | Distal (>10kb) Multiome | 769 | |
| chr11:124,920,725–124,921,671 | 247.2 kb | Distal (>10kb) Multiome | 329 | |
| chr11:124,953,809–124,954,493 | 280.2 kb | Distal (>10kb) Multiome | 818 |
Genomic view of the SPA17 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.