Transcription factors with Perturb-seq knockdown data for SEMA3F-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SEMA3F-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SEMA3F-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:50,154,437–50,155,741 | 278 bp | At TSS | 450 | |
| chr3:50,155,742–50,156,220 | at TSS | At TSS | 164 | |
| chr3:50,157,218–50,157,451 | 1.2 kb | Proximal (<10kb) | 112 | |
| chr3:50,160,572–50,161,312 | 4.6 kb | Proximal (<10kb) | 121 |
Genomic view of the SEMA3F-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.