Transcription factors with Perturb-seq knockdown data for SAP30-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SAP30-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SAP30-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:173,167,923–173,170,542 | 199.9 kb | Distal (>10kb) Multiome | 816 | |
| chr4:173,333,360–173,335,855 | 34.3 kb | Distal (>10kb) Multiome | 1085 | |
| chr4:173,369,270–173,372,372 | 1.1 kb | Proximal (<10kb) Multiome | 989 | |
| chr4:173,448,962–173,449,725 | 79.5 kb | Distal (>10kb) Multiome | 186 | |
| chr4:173,521,529–173,524,247 | 152.7 kb | Distal (>10kb) Multiome | 413 |
Genomic view of the SAP30-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.