RNASEH2A

ribonuclease H2 subunit A | AGS4, RNASEHI, RNHIA, RNHL

The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.[provided by RefSeq, Aug 2009]

Biological processes 21 terms

DNA replication (GO:0006260)RNA binding (GO:0003723)RNA catabolic process (GO:0006401)RNA catabolic process (GO:0006401)RNA metabolic process (GO:0016070)RNA nuclease activity (GO:0004540)RNA-DNA hybrid ribonuclease activity (GO:0004523)RNA-DNA hybrid ribonuclease activity (GO:0004523)RNA-DNA hybrid ribonuclease activity (GO:0004523)cytosol (GO:0005829)mismatch repair (GO:0006298)mismatch repair (GO:0006298)mismatch repair (GO:0006298)nucleic acid binding (GO:0003676)nucleoplasm (GO:0005654)nucleus (GO:0005634)protein binding (GO:0005515)ribonuclease H2 complex (GO:0032299)ribonuclease H2 complex (GO:0032299)ribonuclease H2 complex (GO:0032299)ribonucleotide excision repair (GO:1990516)

Expression (TPM)

TFs regulating RNASEH2A 0 TFs

Transcription factors with Perturb-seq knockdown data for RNASEH2A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RNASEH2A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to RNASEH2A

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RNASEH2A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr19:12,550,951–12,551,971	255.1 kb	Distal (>10kb) Multiome	976
chr19:12,610,516–12,611,613	195.7 kb	Distal (>10kb) Multiome	925
chr19:12,639,899–12,640,614	166.3 kb	Distal (>10kb) Multiome	454
chr19:12,647,538–12,648,022	158.8 kb	Distal (>10kb) Multiome	126
chr19:12,656,871–12,657,566	149.4 kb	Distal (>10kb) Multiome	254
chr19:12,666,317–12,667,225	139.8 kb	Distal (>10kb) Multiome	495
chr19:12,681,003–12,682,117	125.2 kb	Distal (>10kb) Multiome	994
chr19:12,696,035–12,696,970	110.0 kb	Distal (>10kb) Multiome	806
chr19:12,702,968–12,703,602	103.3 kb	Distal (>10kb) Multiome	121
chr19:12,721,692–12,723,084	84.0 kb	Distal (>10kb) Multiome	807
chr19:12,723,726–12,724,142	82.5 kb	Distal (>10kb) Multiome	539
chr19:12,734,221–12,735,284	71.8 kb	Distal (>10kb) Multiome	882
chr19:12,736,470–12,737,780	69.4 kb	Distal (>10kb) Multiome	742
chr19:12,757,454–12,758,297	48.8 kb	Distal (>10kb) Multiome	588
chr19:12,765,876–12,766,545	40.3 kb	Distal (>10kb) Multiome	557
chr19:12,774,659–12,776,025	31.1 kb	Distal (>10kb) Multiome	803
chr19:12,777,481–12,778,589	28.7 kb	Distal (>10kb) Multiome	661
chr19:12,789,543–12,790,654	16.6 kb	Distal (>10kb) Multiome	717
chr19:12,790,960–12,793,231	15.0 kb	Distal (>10kb) Multiome	1015
chr19:12,793,340–12,794,240	12.8 kb	Distal (>10kb) Multiome	808
chr19:12,796,584–12,797,024	9.6 kb	Proximal (<10kb)	479
chr19:12,801,120–12,802,290	4.7 kb	Proximal (<10kb) Multiome	901
chr19:12,806,212–12,806,984	8 bp	At TSS Multiome	827
chr19:12,825,565–12,826,186	19.2 kb	Distal (>10kb) Multiome	436
chr19:12,832,484–12,833,101	26.1 kb	Distal (>10kb) Multiome	970
chr19:12,833,638–12,834,477	27.4 kb	Distal (>10kb) Multiome	310
chr19:12,838,114–12,838,869	31.9 kb	Distal (>10kb) Multiome	377
chr19:12,840,071–12,841,485	34.3 kb	Distal (>10kb) Multiome	391
chr19:12,847,633–12,848,090	41.2 kb	Distal (>10kb) Multiome	535
chr19:12,867,327–12,868,022	61.1 kb	Distal (>10kb) Multiome	315
chr19:12,872,968–12,873,990	66.6 kb	Distal (>10kb) Multiome	263
chr19:12,880,834–12,881,875	74.9 kb	Distal (>10kb) Multiome	656
chr19:12,884,836–12,886,381	78.7 kb	Distal (>10kb) Multiome	717
chr19:12,890,614–12,891,603	84.4 kb	Distal (>10kb) Multiome	841
chr19:12,913,054–12,913,584	106.7 kb	Distal (>10kb) Multiome	373
chr19:12,919,018–12,919,719	112.8 kb	Distal (>10kb) Multiome	732
chr19:12,933,441–12,934,086	127.2 kb	Distal (>10kb) Multiome	789
chr19:12,937,426–12,939,166	131.9 kb	Distal (>10kb) Multiome	943
chr19:12,945,275–12,946,647	139.4 kb	Distal (>10kb) Multiome	1015
chr19:12,956,801–12,957,360	150.6 kb	Distal (>10kb) Multiome	747
chr19:12,964,998–12,965,862	158.9 kb	Distal (>10kb) Multiome	733
chr19:12,983,506–12,983,997	177.1 kb	Distal (>10kb) Multiome	688
chr19:13,051,485–13,052,169	245.2 kb	Distal (>10kb) Multiome	649
chr19:13,061,195–13,061,672	254.9 kb	Distal (>10kb) Multiome	544
chr19:13,093,620–13,094,260	287.3 kb	Distal (>10kb) Multiome	411
chr19:13,095,079–13,096,870	288.9 kb	Distal (>10kb) Multiome	501

Genome Browser

Genomic view of the RNASEH2A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.