This gene encodes an enzyme which catalyzes the translocation of the Man(5)GlcNAc (2)-PP-Dol intermediate from the cytoplasmic to the luminal side of the endoplasmic reticulum membrane in the pathway for the N-glycosylation of proteins. Mutations in this gene are associated with congenital disorder of glycosylation type In.[provided by RefSeq, Dec 2008]
Transcription factors with Perturb-seq knockdown data for RFT1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RFT1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RFT1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:52,897,094–52,897,995 | 232.8 kb | Distal (>10kb) Multiome | 474 | |
| chr3:53,044,987–53,047,058 | 85.0 kb | Distal (>10kb) Multiome | 653 | |
| chr3:53,072,497–53,073,757 | 57.5 kb | Distal (>10kb) Multiome | 597 | |
| chr3:53,130,008–53,130,811 | 32 bp | At TSS Multiome | 781 | |
| chr3:53,134,771–53,135,004 | 4.3 kb | Proximal (<10kb) | 274 | |
| chr3:53,160,572–53,162,503 | 30.5 kb | Distal (>10kb) Multiome HiCAR | 627 | |
| chr3:53,216,604–53,217,511 | 86.5 kb | Distal (>10kb) Multiome | 92 | |
| chr3:53,219,753–53,220,316 | 89.6 kb | Distal (>10kb) Multiome | 367 | |
| chr3:53,254,585–53,256,437 | 125.6 kb | Distal (>10kb) Multiome | 773 | |
| chr3:53,269,991–53,270,526 | 139.8 kb | Distal (>10kb) Multiome | 744 | |
| chr3:53,347,016–53,348,084 | 217.2 kb | Distal (>10kb) Multiome | 1111 |
Genomic view of the RFT1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.