Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009]
Transcription factors with Perturb-seq knockdown data for RFK. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RFK upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RFK, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:76,369,281–76,370,022 | 24.8 kb | Distal (>10kb) Multiome | 236 | |
| chr9:76,385,323–76,385,479 | 8.9 kb | Proximal (<10kb) | 11 | |
| chr9:76,393,705–76,395,262 | 39 bp | At TSS Multiome | 993 | |
| chr9:76,456,070–76,456,805 | 62.0 kb | Distal (>10kb) Multiome | 496 | |
| chr9:76,458,756–76,460,917 | 66.1 kb | Distal (>10kb) Multiome | 663 | |
| chr9:76,490,410–76,491,300 | 96.4 kb | Distal (>10kb) Multiome | 143 | |
| chr9:76,662,490–76,663,545 | 268.7 kb | Distal (>10kb) Multiome HiCAR | 268 |
Genomic view of the RFK locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.